Morphology and DNA marker for distinguishing Paphiopedilum hangianum and Paphiopedilum emersonii from Vietnam

Genus Paphiopedilum has species having lovely flowers which are incredibly attractive to everyone. Their ornamental and commercial value caused over-collection illegal poaching trade. Due these reasons, nowadays, the Venus slipper orchids facing deplete in nature. Therefore, it is important consider conservation. Mainly, necessary prioritize identification phylogenetic analysis methods of genus includes many with similar morphological characteristics. Consequently, isn't easy distinguish identical this when plants young or not yet fully flowering. study aimed two species, i.e. P. hangianum emersonii, have characteristics, through comparative differences DNA barcoding sequences. To solve problem associated identifications, a comparison table was created four sequence markers matK, rbcL, rpoC1 trnH-psbA. The results showed that emersonii significantly different from each other flower's While difference leaf morphology both selected found very little, also distinguishable upon careful comparison. Moreover, indicator gave accurate rapid distinctions between even without flowers. Furthermore, can establish an evolutionary relationship Paphiopedilum. suggested trnH-psbA suitable marker for distinguishing be applied Vietnam.